Promega powerplex y23 pdf free

The powerplex fusion 6c is a sixcolor, 27locus multiplex system. Jul 12, 2012 promega corporation announces the newest addition to the powerplex str systems family, the powerplex y23 systema rapid human identification yshort tandem repeat ystr assay for forensic casework, offender databasing and relationship testing. This standard ensures minimal risk of human dna contamination for products used to collect, store and analyze biological materials for forensic purposes. Promega forensic products are manufactured in alignment with the iso 18385 standard. All primer pairs, with the exception of the se33 primer pairs, are identical in both systems. All amplification protocols outlined in the powerplex y23 user manual were followed unless. Maleline relatives were typed using promega powerplex y23 and for snps defining the main european yhaplogroups in leicester with a subset of the typing being confirmed at.

Promega amplification protocol recommends a pcr total reaction volume of 25. Promega corporation a single multiplex for multiple applications single multiplex containing autosomal loci 2324, y str loci 23 and the mtdna control region equivalent to powerplex fusion, powerplex y23 and mitochondrial control region in a single amplification powerseq mito system. This component should be moved to the postamplification box after opening. Use of the powerplex y23 system to amplify dna from swabs instructions for use of products dc2305 and dc2320. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516. Shakespeare said, the evil that men do lives after them. Powerplex y23 system reproducibly amplifies trace template amounts as. The internal lane standard, wen internal lane standard 500 ess, is designed and optimized specifically for this system. The powerplex 21 system improves on the powerplex 16 hs system performance for even greater inhibitor resistance figure 2, resulting in more interpretable data and less need for reamplification of samples previously deemed difficult due to the presence of inhibitors. Jan 30, 2015 promega has released the powerplex fusion 6c system for forensic casework samples. This is continually true in the case of unidentified remains throughout the united stat. Learn how to interpret results from ystr dna typing using the ychromosome haplotype reference database yhrd, a free resource.

The powerplex esx 17 system is compatible with the abi prism 310, 3100 and 3100avant genetic analyzers and applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers. The purpose of the present study is to validate the powerplex y23 system using half volume reaction mix for the amplification of a wide range of forensic samples. The powerplex y23 system allows ystr analysis of human forensic and database samples. Powerplex y23 system is a 23loci, 5color ystr multiplex designed for genotyping of database, paternity and forensic casework samples. Validation and implementation of the promega powerplex y23. Biallelic polymorphism of ag variation at position 308 in the. The 5x ampsolution reagent, swabsolution kit and punchsolution kit allow fast and simple processing of swabs and punches for analysis with various powerplex systems, including the powerplex fusion, 21, 18d, 16 hs, esx, esi, cs7 and y23 systems.

Use of the powerplexr y23 system to amplify extracted dna. The powerplex y 23 system promega is a short tandem repeat str multiplex that allows coamplification of 23 gonosomal ystrs, combining 17 loci commonly included in commercially available kits dys389i, dys448, dys389ii, dys19, dys391, dys438, dys437, dys635, dys390, dys439, dys392, dys393, dys458, dys385ab, dys456, and ygatah4 and six new loci dys481, dys549, dys533, dys643, dys576. Analysis of powerplex systems using the applied biosystems 3500 and 3500xl genetic analyzers. Powerseq autoy system promega corporation, a multiplex forensic kit for mps, enables analysis of the 22 autosomal str markers plus amelogenin from the powerplex fusion 6c kit and 23 ystr markers from the powerplex y23 kit. Wsp cld forensic dna continuing education day june 18, 2015. Analysis of powerplex systems using the applied biosystems. Developmental validation of the powerplex fusion system for. Validation of powerplex y23 system promega using reduced reaction volume. Manual dexterity definition of manual dexterity by the free dictionary. Validation and implementation of the promega powerplex y23 str system marla kaplan dna technical leader.

Validation and implementation of the promega powerplex. Strengths and limitations of ngs for forensic dna analysis. The powerplex esi 17 pro system is an update to the powerplex esi 17 system. A fast, sensitive and reliable ystr multiplex system for forensic and population genetic purpose article pdf available june 2016 with 380 reads how we measure reads. Add 2l of swab extract to 23l of pcr amplification mix. Recently launched ystr kit, powerplex y23 pp y23 system is a 5dye. Feb 11, 2015 promega corporation announces the release of the powerplex fusion 6c system, a 6color, 27locus multiplex. Haplotype data and mutation rates for the 23 ystr loci of.

The kit contains all 12 loci in powerplex y system, the additional 5 loci found in ampf l str yfiler, plus 6 new loci. Launched in september 2012, the powerplex fusion system is a 24locus multiplex that incorporates common and informative loci used throughout the world. Both kits started exhibiting dropout at approximately 0. The powerplex 16 system is a multiplex str system for use in dna typing, including paternity testing, forensic dna analysis, human identity testing and tissue culture strain identification. It combines common and informative loci to overcome typical challenges faced by laboratories, including sample inhibitors, low levels of input dna, degradation, and mixtures, promega said. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3. Assessment of autosomal and male dna extracted from casework. Promega new powerplexr y23 str system reveals more ystr.

Over the past few years, promega corporation, applied biosystems now life. The 8, 11 genotype was generated using the powerplex 16 and minifiler systems. Of 21 strs in powerplex 21 system promega, usa, 19 loci. A deletion is suspected between the primer binding sites of the two sets. New york genomeweb news promega said today that its powerplex y23 system has been approved for use in laboratories generating dna records for the national dna index system. Validation of powerplex y23 system promega using reduced. Functional validation of humanspecific powerplex 21 system. Apr 12, 2017 todays promega connections blog is written by guest blogger rachel h. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. For the positive amplification control, vortex the tube of 2800m dna, then dilute an aliquot to 2. The powerplex 16 system allows coamplification and threecolor detection of sixteen loci fifteen str loci and amelogenin. Protocol for amplification and analysis of dna from. Promega offers a range of products for cell biology and drug discovery, many of which are built upon bioluminescence technology.

Haplotype data for 23 ychromosome markers in a reference. The system contains the loci penta e, d18s51, d21s11, th01, d3s58, fga, tpox, d8s1179, vwa, amelogenin, penta d, csf1po, d16s539, d7s820, ds317 and d5s818. The powerplex y23 system provides all materials necessary to amplify ystr regions of human. The powerplex y23 system also is compatible with direct amplification, enabling streamlined ystr databasing efforts. The powerplex y23 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block. Thermal cycling time is cut roughly in half, and the system detects substantially more ystr. Protocols for dna amplification using the powerplex y23 system. In this technical tips article, dawn rabbach answers your frequently asked questions about how to analyze powerplex reactions on the new applied biosystems 3500 and 3500xl genetic analyzers. Validation of powerplex y23 pcr amplification system for. Promega was an early supplier in the cell free protein synthesis field and is continuing to develop its portfolio in this area.

Powerplex fusion 6c system a rapid 27plex, including se33, suitable for casework and databasing testing and designed to meet the new codis recommendations powerplex y23 system malespecific str genotyping kit with 23 ystr loci. Promega powerplex y23 system receives ndis approval. Powerplex y23 system, which includes six additional ychromosome strs beyond the. The 5dye str multiplex amplifies 23 ystr loci, including two rapidly mutating ystr loci. Genetic assessment of freeranging and captive populations is the key step. The powerplex y23 system is designed for use with the geneamp pcr system 9700 with a silver or goldplated silver sample block with max mode as the ramp speed. Quick 2prtc thermal cycling use of the powerplex y23 system to amplify dna from storage card punches nstructions for use of products dc2305 and dc2320. Maxwell, plexor, powerplex and powerquant are registered trademarks of promega. The powerplex y23 system is a 23loci, 5color ystr multiplex designed for genotyping forensic casework samples, database samples and paternity samples. Jan 01, 20 the prototype powerplex y23 system promega corporation, madison, wi is a polymerase chain reactionbased amplification kit that targets the 23 y str loci dys19, dys385ab, dys389i, dys389ii, dys390, dys391, dys392, dys393, dys437, dys438, dys439, dys448, dys456, dys458, dys481, dys533, dys549, dys570, dys576, dys635, dys643, and ygatah4.

In the second sample, sequencing confirmed 8 and 11 alleles at d7s820. Identification of the remains of king richard iii nature. American medical association, manual of style, 10th edition, 2007. Mar 22, 20 this approval of powerplex fusion follows the recent ndis approval of powerplex y23 announced in january. The powerplex y23 system allows ystr analysis of human forensic and. Promega powerplex fusion 6c system the scientist magazine.